Viral Gene Delivery Technology

Pre-Made shRNA Lentivirus Particles (SH3001)

The pre-made shRNA expression Lentivirus particle (SH3001) from ATCGbio Life Technology is recombinant shRNA expression lentivirus particle solution to be ready to infect directly for knocking-down gene of interest. It’s specifically designed for scientists who don’t have time or don’t prefer to make lentivirus by themselves. It’s the easiest way to knockdown gene of interest.

We provide only one function-validated shRNA lentivirus particle for each target. The effect of knocking down is guaranteed (up to or more than 70%). If the user needs more than one shRNA sequence for same target, please contact us at info@abdia.com.

Catalogue # shRNA Lentivirus Plamid Species and Gene ID Size Price (USD)
SH3001-01 sh-LTviral-BCL6 (B-Cell CLL/lymphoma 6) Human, Gene ID 604 9.0 mL $350.00
SH3001-10 sh-LTviral-BRF1 Human, Gene ID 2972 9.0 mL $350.00
SH3001-02 sh-LTviral-CAB39 (Calcium binding protein 39) Human, Gene ID 51719 9.0 mL $350.00
SH3001-11A sh-LTviral-CEBPA (CCAAT/enhancer binding protein, alpha) Human, Gene ID 1050 9.0 mL $350.00
SH3001-11B sh-LTviral-CEBPB (CCAAT/enhancer binding protein, beta) Human, Gene ID 1051 9.0 mL $350.00
SH3001-11D sh-LTviral-CEBPD(CCAAT/enhancer binding protein, delta) Human, Gene ID 1052 9.0 mL $350.00
SH3001-12 sh-LTviral-CREBBP (CREB binding protein) Human, Gene ID 1387 9.0 mL $350.00
SH3001-03 sh-LTviral-FOXO3 (Forkhead box O3) Human, Gene ID 2309 9.0 mL $350.00
SH3001-09m sh-LTviral-Mmp3 (Matrix Metallopeptidase 3) Mus musculus, Gene ID 17392 9.0 mL $350.00
SH3001-04 sh-LTviral-PRDM1 (PR domain containing 1, with ZNF domain) Human, Gene ID 639 9.0 mL $350.00
SH3001-05 sh-LTviral-PRKAA1 (AMP-activated, alpha 1 catalytic subunit) Human, Gene ID 5562 9.0 mL $350.00
SH3001-05h2 sh-LTviral-PRKAA2 (AMP-activated, alpha 2 catalytic subunit) Human, Gene ID 5563 9.0 mL $350.00
SH3001-06 sh-LTviral-RUNX1 (Runt-related transcription factor 1) Human, Gene ID 861 9.0 mL $350.00
SH3001-07 sh-LTviral-SIRT1 (sirtuin 1) Human, Gene ID 23411 9.0 mL $350.00
SH3001-07m1 sh-LTviral-mSirt1 (sirtuin 1) Mus musculus, Gene ID 937595 9.0 mL $350.00
SH3001-07h2 sh-LTviral-SIRT2 (sirtuin 2) Human, Gene ID 22933 9.0 mL $350.00
SH3001-08 sh-LTviral-STK11 (Serine/threonine kinase 11, LKB1) Human, Gene ID 6794 9.0 mL $350.00
SH3001-08m sh-LTviral-Stk11 (Serine/threonine kinase 11, Lkb1) Mus musculus, Gene ID 20869 9.0 mL $350.00

Features

shRNA-Lenti particle solutions from AbDia Technologies include two products of categories: Pre-made shRNA-Lenti particle solution (SH3001) and Custom-made shRNA-Lenti particle solution (SH4001).The highlight features of the products are:

  1. Most advanced shRNA design. Near 100% success rate (Fig.1).
  2. High infectious efficiency (Fig.2), up to 0.9 x 108 TU/ml.
  3. Co-express GFP and hygromycine resistance gene.
  4. Just add and incubate overnight (16 hrs).
  5. No cytotoxicity. Small animal injectable without immonoreactive symptoms.
  6. Unlike siRNA, transduced cells show knock-down effects even in divided cells.
  7. No need to add polybrene. Near 100% transduction is possible.
  8. Cell-line can be made in a week.
  9. Suitable for both primary cells and transformed cells.

Contents and Storage

  Catalog # Components Size Storage and Shipping Condition
  SH3001-xxxx Pre-made shRNA Lentivirus particle 9.0ml, up to 0.9 x 108 TU/ml
 Shipped at room temperature, store at -20 °C
  SH3002 shRNA Lentivirus particle (control) 9.0ml, up to 0.9 x 108 TU/ml Shipped at room temperature, store at -20 °C
  SH3003 Lenti-Storage Solution(reconstitution) 1.0ml 
 Shipped at room temperature, Keep at 4°C

Notes

  1. The total price for the first order is $350.00.
  2. For your 2nd order, the same target and control only costs $200.00. Please contact us at info@abdia.com, and fill-in your fist-time order number, catalog number, order date and contact information including name, email, phone and address. We will provide you with a second order code.
  3. We provide only one shRNA targeted lentiviral particle for each target. The effect of knocking down is guaranteed (>70%). If user needs more shRNA sequences for the same target, please at info@abdia.com.
  4. Pre-made shRNA lentivirus particle will be prepared freshly and shipped in 5-7 business days. The shippment method is FedEx priority. The shipment on Friday is not scheduled.
  5. Our lentivirus particle will be shipped in propriety solution making high transduction without polybrene and unprecedented stability:
    • Virus solution can be stored at -20 °C.
    • Allow freezing and thawing up to 2 times.
    • Be stable in room temperature at least 3-4 days.
    • If necessary (the cells are incompatible with DMEM base medium), purification is easy as just centrifuge for 30min at 1500 × g.

References

  1. Y. Ido, et al. PLoS ONE, 2012 Apr 07(4): e35092

  2. Lan F, et al. J Biol Chem. 2008 Oct 10;283(41):27628-35