pLenti™ Lentivirus Production Kit

Catalogue #	Name	Description	Price (USD)
LT1001	pLenti™ Lentivirus Production Kit	3×preparations*	$400.00

* 3 preparations means that the amount of reagents are sufficient to make lentivirus in three 6-well plates. One well of 6-well plate will produce 4ml lentivirus at titer up to 0.9 x 10⁸ TU/ml. 4ml lentivirus are enough for experiments in two 6-well plates at highest amount of lentivirus added (300μl lentivurs per well). At this amount of lentivirus, the primary cells or differentiated cells will be infected more than 70% (the most of those cells will be 90% infected). Some primary cells or differentiated cells just require 50-100μl lentivirus per well to get sufficient infection (>70%).

Features

• Small scale (6 well plate format), high titer (up to 0.9 x 108 TU/ml) virus production system, which in turn to save physical and financial expenditures.


• The provided production medium does not contain chemicals used to add lentivirus production such as sodium butyrate, caffeine, or chloroquine. So, lentivirus in this medium can be directly used for most of applications.


• Reagents for concentration, purification and long-term storage are included.


• The kit produces high quality virus, meaning that higher percentage of virus is functional, resulting in higher transduction rate. High quality of virus is more important than virus number (assessed by p24 antigen, RT-PCR).


• Purified virus can be injectable locally in experimental animals.


• Compatible with both 2nd. and 3rd. generation of virus constructs.


• The kit includes GFP expressing lentivirus plasmid.

Kit Components and Storage Conditions

Catalogue #	Component	Size	Storage Condition
LT1001-1	Lentivirus production medium	55mL	4 °C. Add 1.2ml FBS before use.
LT1001-2	Helper-plasmids mixture	0.2mL	4 °C
LT1001-3	Production helper-solution	0.5mL	4 °C
LT1001-4	Plate coating solution	2.0mL	4 °C
LT1001-5	Lentivirus Purification Solution	20mL	4 °C
LT1001-6	Lentivirus storage solution	2.0mL	4 °C
LT1001-7	GFP expressing lentivirus plasmid	1µg in 20µl TE	4 °C. Ampicillin resistance

References

1. Y Ido, et al. PLoS ONE, 2012 Apr 07( 4): e35092


2. Lan F, et. al. J Biol Chem. 2008 Oct 10;283(41):27628-35