Adenovirus Kits

To inquire about our Adenovirus Kits, contact us here

AbDia Technologies Inc. offers Adeno-Purification^TM Kits that are made for users requiring in-vitro or in-vivo experiments with high quality adenovirus. The combination of AdenoBooster^TMSolution with purified adenovirus enhances in-vivo and in-vitro infection to rodent cells.

1. AdenoComplete^TM kit (AD1001 and AD1002) for both new and experienced users. AD1002 provides GFP expressing adenovirus.
2. AdenoEssential^TM kit (AD1003) is for experienced and cost-concious users.

Catalogue #	Name	Description	Price (USD)
AD1001	AdenoCompleteTM kit	3×preparations	$275.00
AD1002	AdenoCompleteTM kit with CrudeAdeno-GFPTM**	3×preparations with CrudeAdeno-GFPTM**	$300.00
AD1003	AdenoEssentialTM kit	9×preparations	$500.00

** CrudeAdeno-GFP^TM (AD1020) is crude adenovirus which expresses GFP protein for use with product AD1002. 

Features

• Obtain high quality adenovirus in 1.5 hours based on membrane filtration. The procedure does not require ultracentrifugation.


• Typically, it only requires HEK293 cell culture in 4 of 10 cm dishes or T75 flasks (can be scaled-up to 9 dishes or flasks) to yield adenovirus up to 1.0 × 1011 pfu. in 2 ml volume.


• Supplied Stabilizer provides unprecedented stability by virtually eliminating freeze/thaw degradation problem up to 10 times.


• Supplied AdenoBooster^TM Solution (AD1012) could increase the infection efficiency (up to 50 times more) for the cells lacking of coxsackie/adenovirus receptor (CAR) or the cells having difficult infection characters.


• The kits provide enough reagents for 3-times (AdenoComplete^TM, AD1001 & AD1002) and 9-times (AdenoEssential^TM, AD1003 ) purification.


• In AdenoComplete^TM (AD1002), we provide CrudeAdeno-GFP^TM (AD1020) as a positive control.


• In-vivo compatibility. The injection of adenoviruses purified by our kits to mouse through the tail vein shows the efficient transduction in the liver without apparent immunoreactions.


• Very economical. We set up the most cost-effective way to purify adenovirus without compromising performance.


• Detailed protocol describes safe preparation and usage.

Kit Components

Catalogue #	AdenoCompleteTM  (AD1001 & AD1002**)	AdenoEssentialTM (AD1003)
AD1001-01	Blunt needle × 3	Blunt needle× 9
AD1001-02	Binding filter × 1	Binding filter × 3
AD1001-03	30mlLoading Buffer × 1	100ml  Loading Buffer × 1
AD1001-04***	60ml Washing Buffer× 1	5×concentrated 60mlWashing Buffer × 1
AD1001-04-5***	60ml Washing Buffer× 1	5×concentrated 60mlWashing Buffer × 1
AD1001-05	15ml Elution Buffer × 1	60ml Elution Buffer × 1
AD1001-06	1.5ml Stabilizer Solution × 1, keep at 4°C	1.5ml Stabilizer Solution × 2, keep at 4°C
AD1012	0.5ml Booster Solution × 1, keep at 4°C	1.5ml Booster Solution × 1, keep at 4°C

**For product AD1002, the kit supplied withCrudeAdeno-GFP^TM (AD1020). CrudeAdeno-GFP^TM(AD1020) will be shipped at room temperature, store at -20°C once received

***Catalog number AD1001-04 is for 1× Washing Buffer, Catalog number AD1001-04-5 is for 5× concentrated Washing Buffer.

Storage

Store at room temperature (15-30 °C) or at 4°C for extended periods of time.

References

1. Cacicedo JM, Yagihashi N, Keaney JF Jr, Ruderman NB, Ido Y.
   Biochem Biophys Res Commun. 2004 Nov 26;324(4):1204-9.


2. Lan F, Cacicedo JM, Ruderman N, Ido Y.
   J Biol Chem. 2008 Oct 10;283(41):27628-35.


3. Cacicedo JM, Benjachareonwong S, Chou E, Yagihashi N, Ruderman NB, Ido Y. 
   Invest Ophthalmol Vis Sci. 2011 Jun 1;52(6):3630-9.