Adenovirus Companion Products

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AdenoRetriever Solution (AD1011)

Recombinant adenovirus purified by traditional ways will loose the titer easily once it is exposed to room temperature, or kept at – 20 °C even for one day,  or repeated freeze-thaw cycle. The ideal storage is a deep-freezer ( – 80°C or liquid nitrogen). Accident in deep-freezer will cause a terrible loss in the virus stock. In those cases, titer of the virus often become too low to be amplified in 293 cells. AdenoRetriever Solution (AD1011) could save these adenoviruses by retrieving the lost titer for enough to be amplified again in 293 cells.

AdenoBooster Solution (AD1012)

Adenovirus enters the cells through the cosakie-adenovirus receptor. The cells expressing a few numbers of the receptors are very difficult to be infected by adenovirus. AdenoBooster Solution (cat# AD1012) mediates both receptor-independent and dependent route for infection and increases the efficiency up to 50-fold. Mix the solution with adenovirus solution 10 minutes before adding to the cells.

Catalogue #	Name	Size	Price (USD)
AD1011	AdenoRetriever™ Solution	0.5mL	$80.00
AD1012	AdenoBooster™ Solution	0.5mL	$50.00

Features

AdenoBooster Solution (AD1012)

• AdenoBooster^TM Solution (AD1012) could increase adenovirus' infection efficiency up to 50-fold in the cells.


• There is no effect on cell differentiation.

Usage

AdenoRetriever Solution (AD1011)

1. Culture 293 cells in a 6-well plate.

2. Make mixture solution (the volume for one well of the 6-well plate) 

    *Adenovirus / AdenoRetrieverTM Solution = 100μl / 8μl, mixture.

3. Incubate it at room temperature for 10 min

4. Add it in the cell culture

AdenoBooster Solution (AD1012)

1. Make mixture: add 1/10th ~ 3/10th volume of AdenoBooster^TM Solution to the adenovirus solution prepared by our kit. 

    e.g. AdenoBooster^TM Solution/Adenovirus= 1μl/10μl ~ 3μl/10μl

    Note: a). Make mixture at just before infection    

              b). It is strongly recommended that Adenovirus is prepared by our adnovirus purification kits 
            (Adeno-Purification^TM Kits; AD1001, AD1002 & AD1003).

2. Incubate it at room temperature for 5-10min 

3. Add it into the cell culture

4. If the virus amount is less than 10 µl, dilute 7~10 fold of AdenoBooster^TM Solution with sterile DPBS just before infection, and add 1:1 volume ratio of AdenoBooster^TM Solution : Virus solution. 

Storage

Store at 4°C.

Notes

AdenoBooster^TM Solution (AD1012) has a little effects on the cells expressing higher number of cosakie-adenovirus receptors such as 293 cells.